|
KMID : 1025520080500010009
|
|
Journal of Animal Science and Technology
2008 Volume.50 No. 1 p.9 ~ p.18
|
|
|
Practical Use of DNA Polymorphisms in the Avian Immunoglobulin Light Chain Constant Domain for Species-specific PCR
|
|
Choi J. W.
Kang S. J.
Park M. S.
Kim Jin-Kyu
Han Jae-Yong
|
|
|
Abstract
|
|
|
|
Species-specific polymorphisms in chicken, pheasant, turkey, and quail were identified by cloning and sequencing of the immunoglobulin constant domain (IgLC). A set of species-specific primers were then designed on the basis of polymorphisms in the IgLC between species, as well as two additional sets of primers for the cytochrome b and tapasin genes, for the purpose of species identification. Together, the primers successfully distinguished specific species from chicken by species-specific PCR. This simple but unambiguous method may be used to screen avian inter-species germline chimeras, which are valuable models for the conservation of endangered species.
|
|
|
KEYWORD
|
|
|
Avian inter-species chimera, Cytochrome b, Immunoglobulin Light chain constant domain, Species-specific PCR, Tapasin
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|